{"id":14658,"date":"2025-04-13T11:28:47","date_gmt":"2025-04-13T11:28:47","guid":{"rendered":"https:\/\/epiboost.web.ua.pt\/?p=14658"},"modified":"2025-04-14T16:41:54","modified_gmt":"2025-04-14T16:41:54","slug":"epibooster-joao-raio-at-csic-for-3-weeks-of-intense-training","status":"publish","type":"post","link":"https:\/\/epiboost.web.ua.pt\/?p=14658","title":{"rendered":"EPIBOOSTER Jo\u00e3o Raio at CSIC for 3 weeks of intense training"},"content":{"rendered":"\n<h6 class=\"wp-block-heading\">Molecular endpoints following the seabass experiments<\/h6>\n\n\n\n<p>Jo\u00e3o Raio, a MSc student supervised by Patr\u00edcia Pereira and Laia Navarro-Mart\u00edn was at IDAEA-CSIC from March 17th to April 11th 2025 to receive training regarding the assessment of gene expression. in seabass following the exposures carried out with the seabass under the scope of EPIBOOST.<\/p>\n\n\n\n<p>Hard work in a short time was ahead of Jo\u00e3o, but the willingness to learn and dedication, together with all the support from the receiving team made this Exchange a successful story and EPIBOOST is proud of it! Under a hands-on approach that characterizes all EPIBOOST exchanges, Jo\u00e3o learned fine-tuned protocols to extract RNA and convert it into cDNA for gene expression quantification through real-time PCR. He managed to run himself real-time PCR assessment for five genes of interest within his MSc thesis scope plus appropriate reference genes. Beyond the wet-lab training, Jo\u00e3o was trained with the receiving team on high-throughput methods for real-time PCR analysis and bioinformatics on whole transcriptome (RNAseq) data. And\u2026 He also presented the results already obtained in Aveiro regarding the oxidative stress effects of cadmium and ciprofloxacin in seabass to the host team in a group meeting.<\/p>\n\n\n\n<p>Thank you Jo\u00e3o for all the hard work and enthusiasm! We are looking forward to see your results and waving for your success! And\u2026 A huge thank you to Laia Navarro-Mart\u00edn and the whole IDAEA-CSIC team for all the sharing and valuable knowledge Exchange.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"375\" src=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR2.jpg\" alt=\"\" class=\"wp-image-14672\" srcset=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR2.jpg 500w, https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR2-300x225.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"375\" src=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR1.jpg\" alt=\"\" class=\"wp-image-14669\" srcset=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR1.jpg 500w, https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-qPCR1-300x225.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"375\" src=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-RNA-analysis1.jpg\" alt=\"\" class=\"wp-image-14675\" srcset=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-RNA-analysis1.jpg 500w, https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Joao-RNA-analysis1-300x225.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"375\" src=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Seminar-Joao-1-55x375-1.jpg\" alt=\"\" class=\"wp-image-14678\" srcset=\"https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Seminar-Joao-1-55x375-1.jpg 500w, https:\/\/epiboost.web.ua.pt\/wp-content\/uploads\/2025\/04\/Seminar-Joao-1-55x375-1-300x225.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Molecular endpoints following the seabass experiments Jo\u00e3o Raio, a MSc student supervised by Patr\u00edcia Pereira and Laia Navarro-Mart\u00edn was at IDAEA-CSIC from March 17th to April 11th 2025 to receive training regarding the assessment of gene expression. in seabass following the exposures carried out with the seabass under the scope of EPIBOOST. Hard work in [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-14658","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/posts\/14658","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14658"}],"version-history":[{"count":3,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/posts\/14658\/revisions"}],"predecessor-version":[{"id":14684,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=\/wp\/v2\/posts\/14658\/revisions\/14684"}],"wp:attachment":[{"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14658"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14658"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/epiboost.web.ua.pt\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14658"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}